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UCT Repository Prague MASTER THESIS

Význam interakcí buněčných povrchů v biodegradačních procesech

Importance of cell surfaces in biodegradation

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Ing. Milena Ouzká
UCT Repository Prague MASTER THESIS

Význam interakcí buněčných povrchů v biodegradačních procesech

Importance of cell surfaces in biodegradation

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Ing. Milena Ouzká

ANOTACE

Bunková stena predstavuje bariéru chrániacu mikroorganizmus pred vplyvmi vonkajšieho prostredia, ktorá zároveň umožňuje transport a komunikáciu medzi okolím a vnútrom bunky. Jej štruktúra hrá významnú úlohu pri medzibunkových interakciách a adhézii na inertné povrchy. Bunkové obaly študovanej baktérie Rhodococcus erythropolis sa vyznačujú atypickou stavbou. Prvá časť práce bola zameraná na výskum vplyvu substrátu na vlastnosti bunkových obalov. Bunky kultivované na glukóze a fenole vykazovali vysoké hodnoty hydrofobity, ktoré významne klesli, keď bol ako substrát využitý anilín. Stresový vplyv fenolu a anilínu sa prejavil zvýšením permeability bunkových stien pre hydrofilné i hydrofóbne zlúčeniny v porovnaní s permeabilitou buniek utilizujúcich glukózu. V rámci experimentov bola sledovaná i degradácia…

ANNOTATION

The cell wall is a rigid structure which provides protection from external environment conditions. It plays important role in cell-cell interactions and adhesion to inert surfaces. The cell envelope of Rhodococcus erythropolis studied in this work exhibits an atypical composition. The first part of this study was focused on characterization of cell envelopes of biomass growing on different substrates. The cells cultivated on glucose and phenol exhibited high values of hydrophobicity. Cultivation on aniline gave cells with low hydrophobicity. Phenol and/or aniline used as single C-sources increased permeability of cells for hydrophilic and hydrophobic compounds. Adhesion and biofilm formation on two materials were studied in batch column systems. Hydrophobic polypropylene was better colonized by R. er…

Advisor
prof. Ing. Jan Masák, CSc.
Referee
RNDr. Tomáš Řezanka, CSc. DSc.
Thesis type
Master thesis
Institution
University of Chemistry and Technology, Prague
Faculty
Faculty of Food and Biochemical Technology
Department
Department of Biotechnology
Study programme
Fermentation Chemistry and Bioengineering
Specialization / field
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MŠMT code
2810x002
Language
Czech
Resource accessibility
The printed version of the thesis is available at the department where the thesis was produced. Defence protocol and reviews are available at UCT Prague archive.
Access rights
open access
SIS ID
3601
Date available
Jun 20, 2007
Date modified
Jun 20, 2007
Date defended
Jun 6, 2007DEFENDED