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UCT Repository Prague BACHELOR THESIS

Výskyt a detekce poly-P baktérií v aktivovaném kalu

Occurrence and detection of poly-P bacteria in activated sludge

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Bc. Hana Šedivá
UCT Repository Prague BACHELOR THESIS

Výskyt a detekce poly-P baktérií v aktivovaném kalu

Occurrence and detection of poly-P bacteria in activated sludge

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Bc. Hana Šedivá

ANOTACE

Cílem této práce bylo detekovat polyfosfátakumulující (poly-P) bakterie rodu Accumulibacter v aktivovaných kalech 5 čistíren odpadních vod (ČOV) s různým technologickým uspořádáním linky. Detekce těchto bakterií probíhala dvěma způsoby, Neisserovým barvením a pomocí metody fluorescenční in situ hybridizace (FISH), což je metoda molekulární biologie. Pro identifikaci druhou zmiňovanou technikou byly aplikovány 3 odlišné sady genových sond (PAO-mix, PAOb-mix, RHC-mix), které se od sebe liší jejich sekvencemi specifickými pro poly-P bakterie. Během mikroskopické analýzy byly pořízeny snímky cílových bakterií, které byly využity pro kvantifikaci, aby následně mohly být porovnány jednotlivé možnosti detekce cílových bakterií. Shluky poly-P bakterií, detekované pomocí Neisserova barvení, byly p…

ANNOTATION

The aim of this study was to detect polyphosphate-accumulating organisms (PAOs) of the genus Accumulibacter in activated sludge from five wastewater treatment plants (WWTPs) with different technological systems. Two methods for a detection were used - Neisser staining and fluorescence in situ hybridization (FISH), which is a method of molecular biology. For the identification by FISH three sets of gene probes (PAO-mix, PAOb-mix, RHC-mix) were applied with different specific sequences for poly-P bacteria. Images acquired during the microscopic analysis were subsequently used for quantification. Clusters of poly-P bacteria detected by Neisser staining were counted manually and photos taken during FISH analyses were processed by the program Daime, which is a special software for that. The re…

Advisor
Ing. Iveta Růžičková, Ph.D.
Ing. Lucie Chovancová
Thesis type
Bachelor thesis
Institution
University of Chemistry and Technology, Prague
Faculty
Faculty of Environmental Technology
Department
Department of Water Technology and Environmental Engineering
Study programme
Environmental Chemistry and Technology
Specialization / field
Enviromental Technology
MŠMT code
2805R002
Language
Czech
Resource accessibility
The printed version of the thesis is available at the department where the thesis was produced. Defence protocol and reviews are available at UCT Prague archive.
Access rights
open access
SIS ID
16214
Date available
Jul 8, 2014
Date modified
Jul 8, 2014
Date defended
Jun 24, 2014DEFENDED